Taiz Zeiger Plant Physiology 5th Edition Free !FREE! 601

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Proximate analysis of food/plant materials for moisture, crude protein, crude fibre, ash, ether extract and nitrogen free extract. Determination of true protein and oil contents of plant materials. Estimation of vitamin-C and pectin in fruit juices. Estimation of glucose, fructose and starch in plant products.

In the growth chamber experiment, elongating shoot tips were harvested after three weeks of starting short day conditions and immediately placed in liquid N2. The samples were freeze-dried using a freeze dryer machine (Heto Holten A/S, Allerød, Denmark). For each genotype, 3 replicate samples, each containing 3 shoot tips (elongating parts of the stem) were used for auxin analysis. The frozen plant materials were ground in a mortar and extracted for 5 min with 1 ml cold phosphate buffer (50 mM, pH 7.0) containing 0.2% sodium diethyldithiocarbomate. 15N and 2H5-labeled internal standards of indole -3-acetic acid (IAA) and IAA metabolites were obtained from OlChemlm (Olomouc, Czech Republic). The measurements were performed in duplicate. The samples were processed according to Pěnčík et al. [44] using high performance liquid chromatography (HPLC) coupled to a tandem triple-quadrupole mass spectrometer (MS/MS). 2b1af7f3a8